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Image Search Results
Journal: The American journal of clinical nutrition
Article Title: Vitamin B-12 and liver activity and expression of methionine synthase are decreased in fetuses with neural tube defects.
doi: 10.1093/ajcn/nqy340
Figure Lengend Snippet: FIGURE 1 Nutritional and metabolic markers and activities and expression of enzymes from the homocysteine remethylation pathway in liver tissue from NTD (open bars) (n = 14) and non-NTD fetuses (black bars) (n = 16). (A) Nutritional markers, folate (B9), vitamin B-12 (B12), and holoTC (nmol/g protein). (B) SAM and SAH (nmol/g protein). (C) Enzymatic activities of CBS, MS, MTHFR, and BHMT (nmol · h–1 · mg protein–1). (D) Gene expression of MTR and MTHFR. The expressions of the 2 genes were normalized using POLR2A as reference gene. Values are means ± SEMs; ∗P < 0.05, ∗∗P < 0.005, ∗∗∗P < 0.0005. BHMT, betaine-homocysteine S-methyltransferase; CBS, cystathionine β-synthase; holoTC, holotranscobalamin; MS, methionine synthase; MTHFR, methylenetetrahydrofolate reductase; MTR, methionine synthase; NTD, neural tube defect; POL2RA, RNA polymerase2 subunitA; SAH, S-adenosyl homocysteine; SAM, S-adenosyl methionine.
Article Snippet: The membranes were incubated overnight at 4◦C with primary antibodies diluted in trisbuffered saline containing 5% nonfat dried milk at the appropriate dilution (BHMT, Abcam, Ab15144;
Techniques: Expressing, Gene Expression
Journal: The American journal of clinical nutrition
Article Title: Vitamin B-12 and liver activity and expression of methionine synthase are decreased in fetuses with neural tube defects.
doi: 10.1093/ajcn/nqy340
Figure Lengend Snippet: FIGURE 2 Western blot (WB) analyses of MS, CBS, MTHFR, and BHMT in fetal liver tissues from NTD and non-NTD fetuses (controls). (A) Examples of western blots. GAPDH was used as reference protein. (B) Densitometric analysis of western blots of MS, MTHFR, BHMT, and CBS normalized with GAPDH, in fetal liver tissues from NTD (open bars) (n = 14) and non-NTD fetuses (black bars) (n = 16). Values are means ± SEMs; ∗∗P < 0.005. BHMT, betaine-homocysteine methyl- transferase; CBS, cystathionine β-synthase; GAPDH, glyceraldehyde-3- phosphate dehydrogenase; MS, methionine synthase; MTHFR, methylenete- trahydrofolate reductase; NTD, neural tube defect.
Article Snippet: The membranes were incubated overnight at 4◦C with primary antibodies diluted in trisbuffered saline containing 5% nonfat dried milk at the appropriate dilution (BHMT, Abcam, Ab15144;
Techniques: Western Blot
Journal: The American journal of clinical nutrition
Article Title: Vitamin B-12 and liver activity and expression of methionine synthase are decreased in fetuses with neural tube defects.
doi: 10.1093/ajcn/nqy340
Figure Lengend Snippet: FIGURE 3 MTR gene transcript level in liver fetal tissue according to the genotypes (AA, n = 21 and AG + GG, n = 9) of polymorphism rs1805087 (A). The lowest level of transcripts is observed for the AG + GG genotype. (B) MS activity in liver fetal tissue according to the polymorphism rs1805087 of MTR. The lowest activity is observed in the AG + AG genotypes of rs1805087. The dotted line represents the threshold of the values reported in control fetuses. Ct, cycle threshold; MS, methionine synthase; MTHFR, methylenetetrahydrofolate reductase; MTR, methionine synthase; NTD, neural tube defect; POL2RA, RNA polymerase2 subunitA; wa, weeks of amenorrhea.
Article Snippet: The membranes were incubated overnight at 4◦C with primary antibodies diluted in trisbuffered saline containing 5% nonfat dried milk at the appropriate dilution (BHMT, Abcam, Ab15144;
Techniques: Activity Assay, Control